Investigating the impacts of Static Magnetic Field on cell viability and Cell Cycle Progression in Human Mesenchymal Stem cells
Maryam
Sadri
Ph.D Student, Biophysics Department, Faculty of Biological Sciences, Tarbiat Modares University
author
Parviz
Abdolmaleki
Associate professor, Biophysics Department, Faculty of Biological Sciences, Tarbiat Modares University
author
Saeed
Abroun
Associate professor, Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University
author
Bahare
Beiki
M.Sc, Royan Stem Cell Technology Company (Royan Cord Blood Bank, R&D section
author
Fazel
Samani
M.Sc, Department of stem cells and developmental biology, cell sciences research center, Royan Institute for Stem Cell Biology and Technology, ACECR
author
text
article
2012
per
The Mesenchymal Stem cells derived from human newborn cords were cultured and exposed to a 24mT Static magnetic field for 24 hours. The viability percentage and the cell cycle progression was then investigated in exposed samples and the obtained results was compared with the control samples. The results clearly demonstrated a significant reduction of cell viability due to the exposure of 24 hours of SMF and post-exposure cultures within the time frames of 36,48,60 hours. The cell development through the cell-cycle, also verified this finding, however, 72 hours of post-exposure culture, significantly leveled off the drop in viable stem cell rates.
Iranian Journal of Biomedical Engineering
Iranian Society for Biomedical Engineering
5869-2008
6
v.
2
no.
2012
91
98
https://www.ijbme.org/article_13103_a171dd15229c8607a0b762644f126326.pdf
dx.doi.org/10.22041/ijbme.2012.13103
Protective effects of saffron extract against consolidation Impairment memory induced by magnetic field in rats
Samane
Sedighi
M.Sc., Department of Biology, Islamic Azad University of Damghan
author
Keyvan
Keramati
Associate professor, Department of Biology, Islamic Azad University of Damghan
author
Ali
Safari-Varyani
Associate professor, Department of Occupational Health, Qazvin University of Medical Sciences
author
Ahmad
Nikpey
Associate professor Department of Occupational Health, Qazvin University of Medical Sciences
author
text
article
2012
per
Exposure to magnetic fields can effect on the learning and memory. The protective effect of saffron extract on memory consolidation disorders in rats exposed to magnetic fields was investigated. 120 male Wistar rats in 12 groups exposed to magnetic field For 5 days with intensity 2.5 tesla for 1, 3 and 5 hour and protective effects of saffron extract with doses of 125 mg, 200 mg and 300 mg (P ≤0.05) compared to the control group by passive avoidance learning method in shuttle box. One hour exposure with magnetic field had no effect on the rats’ memory consolidation (P ≤0.05). Increase exposure time to 3 and 5 hours had a memory consolidation Impairment compared to the control group (P ≤0.05). Administered rats with 300 mg Inter peritoneal saffron extract improved memory consolidation (P ≤0.05) compared to the control group. Exposure to magnetic fields 2.5 mT, 50 Hz impair memory consolidation. Saffron aqueous extract at a dose of 300 mg per kg may have a protective effect and be improvement consolidation impairments.
Iranian Journal of Biomedical Engineering
Iranian Society for Biomedical Engineering
5869-2008
6
v.
2
no.
2012
99
106
https://www.ijbme.org/article_13105_5197cf895b4c162b56e6e65b121a0bc6.pdf
dx.doi.org/10.22041/ijbme.2012.13105
The Effect of Extremely Low Frequency Pulsed Electromagnetic Field on anxiety and cortisol level in rat
Susan
Kohzad
M.Sc, Department of Medical Physics and Engineering, Tehran University of Medical Sciences
author
Bahram
Bolouri
Assistant Professor, Department of Medical Physics, Iran University of Medical Sciences
author
Farnaz
Nikbakht
Assistant Professor ,Physiology Research Center, College of Medicine, Iran University of Medical Sciences
author
Zahra
Kohzad
M.Sc, Department of Psychology, Islamic Azad University of Ilam
author
text
article
2012
per
There is a growing public concern that the extremely low frequency (ELF) range of the environmental electromagnetic fields may have adverse biological effects. In this frequency range, 217Hz is the modulating signal being used in Global System of Mobile. This study investigated the possible effects of 217 Hz pulsed electromagnetic field on the anxiety and the cortisol level in rats. Twenty four male Wistar rat (200 - 250 g) were randomly grouped into test, sham and control. Using a pair of Helmholtz coil system, the test group was exposed to a uniform pulsed EMF of 200µT intensity for 4 h/day for 21 days. A similar procedure with no field was repeated for the sham group. All groups were tested in an `Elevated- plus` maze system. Then via the heart puncture scheme, the blood samples were collected. The serum cortisol levels were evaluated using ELISA method.The ANOVA test revealed no significant differences for the Elevated- plus maze test. Serum cortisol level was significantly higher in test group compared to the control group.These findings were in consistent with the work of others indicating that low frequency band of EMF might not have any effect on the anxiety but it increases the cortisol levels as a stress marker.
Iranian Journal of Biomedical Engineering
Iranian Society for Biomedical Engineering
5869-2008
6
v.
2
no.
2012
107
111
https://www.ijbme.org/article_13107_6971d0aa0633efff0d9ec1ffa2cd89e0.pdf
dx.doi.org/10.22041/ijbme.2012.13107
Stimulation of Taxol Production by Magnetic Field in Cell Culture of Hazel (CorylusavellanaL.)
Ayatollah
Rezaei
Assistant Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, Shahed University
author
Faeze
Ghanati
2Associate Professor, Department of Plant Biology, Faculty of Biological Science, TarbiatModares University
author
Mehrdad
Behmanesh
Associate Professor, Department of Genetics, Faculty of Biological Sciences, TarbiatModares University
author
text
article
2012
per
In this study cell growth, some physiological parameters, production of Taxol and gene expression in cell culture of hazel under effect of the magnetic field were investigated. Cells in suspension culture were treated by a 30 mT static magnetic field on days 8-11 after subculture and 4 hours each day. The results showed that while the growth rate and viability of cells weren’t affected by the magnetic field but membrane lipid peroxidation rate and H2O2 production increased. Activity of phenylalanine ammonia lyase, polyphenol oxidase and peroxidase enzymes was increased by the magnetic field compared with control. Production of phenolic compounds and Taxolin treated cells showed an increase compared to those of control cells. Magnetic field increased intracellular Taxol more than extracellurTaxol, and in treated cultures total taxol production was 2.9-fold compared to control culture. Gene expression of 1- deoxy -D- xylulose -5 - phosphate reductoisomerase involved in producing Taxol precursors and in its biosynthesis was also increased in treated cells compared to control. It appears that magnetic field by stimulating cell defense responses and inducing gene expression involved in Taxol biosynthesis has resulted in improved its production.
Iranian Journal of Biomedical Engineering
Iranian Society for Biomedical Engineering
5869-2008
6
v.
2
no.
2012
113
122
https://www.ijbme.org/article_13108_fa113c844cff69c676b785df4d31a150.pdf
dx.doi.org/10.22041/ijbme.2012.13108
The effect of ELF Magnetic fields on Nonlinear features of Helix Aspersa’s Neurons
Hadi
Tavakoli
Ph.D Student, BioMedical Engineering Department, Faculty of Engineering, Shahed University
author
Ali
Motie Nasrabadi
Associate Professor, BioMedical Engineering Department, Faculty of Engineering, Shahed University
author
Seyed Mohammad
Firouzabadi
Professor,Medical Physics Department, Faculty of Medical Sciences, Tarbiat Modares University
author
Mehri
Kaviyani Moghaddam
Medical Physics Department, Faculty of Medical Sciences, Tarbiat Modares University
author
text
article
2012
per
During recent years, the environment has been enormously changed by the wide range of magnetic fields. Therefore, comprehensive studies are being done for investigating their biological effects. The effects such as inhibition of bioelectric activity of neurons which is shown by evidence, like decreasing in the firing frequency or decreasing in the amplitude of action potential, have been shown. To notify and investigate these effects, the theory of “biological windows” have been proposed and considered. The effects of amplitude and/or frequency of magnetic field have been pointed in some research. In this study, regarding the behavior of nervous system, which has non-linear dynamic behavior, we study the behavior of nervous system under exposure to magnetic field. We investigate whether the low frequency field is able to affect the dynamic of nerve cells and to have influence on non-linear features of signal. We used 6 environmental intensities and 6 cells have been used in each intensity, and by calculating some of non-linear features of action potential such as Higuchi Dimension and Return map of signal, during the time and in some different intensities of magnetic fields, It was observed that all intensities magnetic fields lead to increasing in Higuchi Dimension and increasing in the scattering of the Return map of signal. Of course these effects has been more observed in the middle band of frequency which has been confirmed by the theory of ‘frequency window’ effect of magnetic fields, which it has been noticed and discussed in last two decades.
Iranian Journal of Biomedical Engineering
Iranian Society for Biomedical Engineering
5869-2008
6
v.
2
no.
2012
123
131
https://www.ijbme.org/article_13110_2cf4f5fba7d3c65f0ec51953deb6011e.pdf
dx.doi.org/10.22041/ijbme.2012.13110
A Novel Approach to Computing Induced Electric Fields in Biological Structures Based on Sequences of Transfer Functions
Mehrdad
Saviz
Post-Doctoral Researcher, Bioelectromagnetics Laboratory, School of Electrical and Computer Eng., University of Tehran
author
Sina
Shirinpour
B.Sc. Student, School of Electrical and Computer Eng., University of Tehran
author
Ashkan
Abedi
B.Sc. Student, School of Electrical and Computer Eng., University of Tehran
author
Reza
Faraji-Dana
Professor, Center of Excellence on Applied Electromagnetic Systems, University of Tehran
author
text
article
2012
per
We introduce a new computational approach which is capable of providing estimations of the electric field strength induced in biological bodies at large to ultra-fine scales. The method is theoretically based on multi-scale analysis and excitation of the smaller-scale models by the computed fields at the larger-scale model. The method and its implementation are shown, and as a practical example, the electric field induced inside the plasma membrane has been successfully computed for cells residing at different locations in the human body-model. Also discussed are the origins of the frequency-dependent behavior of the induced field strength and the significance of its practical consequences for bioelectromagnetics.
Iranian Journal of Biomedical Engineering
Iranian Society for Biomedical Engineering
5869-2008
6
v.
2
no.
2012
133
140
https://www.ijbme.org/article_13111_cfd65321ea10971ea00b0aa320bc5dec.pdf
dx.doi.org/10.22041/ijbme.2012.13111
Foot movement onset detection in self-paced BCIs using sparse representation based classifier
Rahele
Mohammadi
PhD student of Biomedical Engineering, Electrical and Computer Eng. College, Tarbiat Modares University
author
Ali
Mahloojifar
Associate Professor, Biomedical Engineering department, Electrical and Computer Eng. College, Tarbiat Modares University
author
text
article
2012
per
Self-paced BCI systems are more natural for real-life applications since these systems allow the user to control the system when desired. Detection of event periods in continuous EEG signal is one of the most important challenges in designing self-paced BCIs. In this paper, the Event related synchronization (ERS) is extracted from idle EEG signal using fractal dimensions in frequency range from 6 to 36 Hz and sparse representation based classifier. Our proposed method applied on EEG signal recorded during executing foot movement in 7 subjects. The average true positive rate and false positive rate equal to 90% and 5% were achieved.
Iranian Journal of Biomedical Engineering
Iranian Society for Biomedical Engineering
5869-2008
6
v.
2
no.
2012
141
152
https://www.ijbme.org/article_13112_f163dd7680e3e640cfbc66e94fcef925.pdf
dx.doi.org/10.22041/ijbme.2012.13112